Principal Investigator
Our main research objective is to resolve the long-standing question: How do cells direct their proteome to fold to their native, functional state and avoid misfolding diseases. We are explorÖ´Ö´Ö´ing the mechanisms guiding the folding and assembly of newly synthesized proteins into multi-molecular complexes as well as the mechanisms for degradation of “lonely” subunits.
We are studying the role of the ribosome as a platform for coordinating complex assembly during synthesis, by advanced techniques combining biochemistry and deep sequencing such as selective ribosome profiling as well as super-resolution microscopy. We are also developing tools for studying single-molecule mRNA-protein interactions, in vivo.
Lab Manager & Research associate
BSc in molecular biology at the Department of Life Sciences, Bar-Ilan University, Israel. Master’s degree at Shimon Pollack`s lab, at the Department of Immunology in the Faculty of Medicine at the Technion-Israel Institute of Technology “Flagellin activation of Toll-Like Receptor 5 directs human monocytes into distinct differentiation towards dendritic cells”.
Phd in Daniel Kornitze`s lab at the Department of Molecular Microbiology in the Faculty of Medicine at the Technion “Genetic and pharmacological suppression of fungalmorphogenesis”. In her work on Candida albicans, she showed that endocytosis is both essential and highly regulated in hyphal morphogenesis. Since 2017 she is working at the Biology Faculty, Technion.
First worked as head of student labs for bachelor’s studies at the faculty.
In October 2019 she joined the lab and is working on co-translational protein folding.
Students
Johannes A. Venezian
Ph.D student (Direct track)
B.Sc. in Physics and Mathematics, Technion, Israel.
Graduation project, under prof. Enrique Kajomovitz’s supervision, Toy Monte Carlo simulation for the production of jets and their reconstruction in the ROOT environment.
Current Research: Evolution of protein folding and assembly pathways: Deciphering the characteristics of divergent co-translational assembly pathways.
Hila Ben-Arie Zilberman
Ph.D. student
B.Sc. in Biotechnology and food engineering, Technion, Israel.
Research: Mechanistic analysis of ISGylation and its components at the ribosome, and ISG15's impact on translation following an innate immune response.
Junyi He
Ph.D student
B.Sc.: College of Plant Protection, Northwest A&F University, China.
Thesis: “GST pulldown assay between the wheat stripe rust effector protein RTP1 and its candidate target protein, Nudix23”.
M.Sc: The Jacob Blaustein Institute for Desert Research, Ben Gurion University of the Negev, Simon Barak`s lab. Thesis: “Molecular mechanisms of long and short term salt mediated inhibition of seed germination of Eutrema salsugineum and Schrenkiella parvula”.
Research: Discovery of assembly-specific degradation factors; a-systematic, proteome-wide identification of novel, co-translationally acting degradation factors.
Qamar Shhade
Ph.D student (Direct track)
email: qamar.shhade.98@gmail.com
B.Sc. in Medical Sciences and Biology, University of Haifa, Israel.
Thesis: “Cytosolic dsDNA emergence following macrophage efferocytosis might lead to STING mediated production of IFN-β during the resolution of inflammation”.
Research: mRNA spatial organization in the cell.
Muhammad Makhzumy
Ph.D. student
email: muhammad@mmcmed.co
B.Sc. in Biotechnology and food engineering, Technion, Israel.
Master of Engineering, Biotechnology and food engineering, Technion, Israel. Protein Purification team lead, Stem Cells and Bioreactors logistics manager, Vaccines production manager and Medical Device Quality Assurance manager.
Research: Single-molecule approaches to ribosome profiling.
Adva Kfir
M.Sc. student
B.Sc. in Biology, Tel Aviv University, Israel.
Graduation project, under prof. Gali Prag's supervision, Split-Chloramphenicol Acetyl Transferase assay to examine possible ubiquitylation substrates for the Nleg6-2 ubiquitin ligase of enterohemorrhagic E. coli.
Rawad Hanna
Post-Doc
B.Sc. in Biochemical Engineering, Technion, Israel.
M.Sc. and PhD in Oded’s Kleifeld lab, Faculty of Biology, Technion, Israel
Specialized in Proteomics method development and data analysis
Current project: Method development for Nascent Proteome characterization
Alumni
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Tamar Gilinski
Project student
Prediction of NatB complex structurein Saccharomyces cerevisiae
Shoham Alhanati
Project student
Structural characterization and Modelling of co-translationally assembling Protein Complexes
Seraj Attarya
M.Sc. student
B.Sc. in Medical Sciences and Biology, Technion, Israel. Thesis: “Myelodysplastic syndrome link to Acute Myeloid Leukemia”.
Research: Discovery of assembly-specific degradation factors. A systematic, proteome-wide identification of novel, co-translationally acting degradation factors.
Maayan Cohen
Project student
As our experienced Research Adviser, Morgan James is a fundamental part of what makes Shiber Lab such a successful Research Lab. They are truly an invaluable member of our hardworking team.
Daniel Dahan
Project student
Data Science & Engineering student.
Project: Computational modeling of co-translational interface folding